Gourmet Liquid Cultures: Storage & Use

Pearl Oyster Liquid Culture 

Storage:

  • Gourmet liquid culture syringes are best stored at room temperature. Because a liquid culture is live mycelium, it’s more susceptible to cold/hot temperatures.
  • Liquid cultures can remain viable for approximately 6 months, but it is best to use them as soon as possible. 
  • DO NOT FREEZE

How To Use:

  • For best results, use in a sterile environment! In front of laminar flow and/or a HEPA filter is ideal, but still air boxes (SAB) or in a small, freshly sterilized room with no air flow can work. Note that there is an increased risk of contamination if not used in a completely sterile environment
    • We make all of our liquid cultures in front of laminar flow hoods with HEPA filter to ensure the cleanest possible products
  • Be sure to wear a mask and gloves when handling liquid culture syringes.
  • Sterilize the entire syringe with 70-85% isopropyl alcohol before using
  • Open needle’s sterile packaging
  • Remove black cap from syringe and then quickly attach needle to the syringe
    • The needle should always be flame sterilized before every use. Let needle cool and then dispense liquid culture into your growing medium
      • It is recommended to use ~1cc of gourmet liquid culture for every 1 pound of substrate